Site Directed Mutagenesis (SDM) Rat Point mutation Rat-2

Get tips on using ErbB2 (HER-2) Antibody Cocktail to perform Western blotting HER2

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using EpiTect 96 Bisulfite Kit (2) to perform Bisulfite DNA Modification Fluids

Get tips on using FaqI (BsmFI) (2 U/µL) to perform Restriction Enzymes BsmFI / FaqI

Get tips on using BspPI (AlwI) (2 U/µL) to perform Restriction Enzymes AlwI / BspPI

Get tips on using TIMP-2 Antibody (B-12): sc-365671 to perform Western blotting TIMP-2

Get tips on using Mouse BMP-2 PicoKine™ ELISA Kit to perform ELISA Mouse - BMP-2

Get tips on using Human BMP-2 PicoKine™ ELISA Kit to perform ELISA Human - BMP-2

Get tips on using MAP LC3β Antibody (G-2) to perform Autophagy assay cell type - AR42J