Protein Expression Prokaryotic cells C. crescentus JS1014

Get tips on using PE anti-mouse/rat CD29 Antibody to perform Flow cytometry Anti-bodies Mouse - CD29/β1-Integrin

Get tips on using CD200 Monoclonal Antibody (OX90), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD200

Get tips on using CD73 Monoclonal Antibody (AD2), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD73

Get tips on using CD73 Monoclonal Antibody (AD2), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD73

Get tips on using Purified NA/LE Hamster Anti-Mouse CD40 to perform Flow cytometry Anti-bodies Mouse - CD40

Get tips on using CD40 Monoclonal Antibody (1C10), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD40

Get tips on using Monoclonal Mouse Anti-Human Androgen Receptor (Concentrate) Clone AR441 to perform Immunohistochemistry Human - AR

Get tips on using Biotin Rat Anti-Mouse OX40 Ligand (CD252) to perform Flow cytometry Anti-bodies Mouse - CD252/OX40L

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.