shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3) CD24

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I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Discussions Problem in phase separation after using serum/plasma kit

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Discussions Is a bacterial nuclease contamination possible during protein purification?

DNA PCR Multiplex PCR Viral

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DNA PCR Conventional / Qualitative PCR V

DNA PCR Conventional / Qualitative PCR V

DNA PCR Conventional / Qualitative PCR Vi

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