Protein Expression

Get tips on using pUC19-Pgpd-xyn2-Tgpd to perform Protein Expression Eukaryotic cells - T. reesei xyn2

Get tips on using OmpA-TRAIL/pET-22b to perform Protein Expression Prokaryotic cells - E. coli TRAIL

Get tips on using pMmEG(TA)-rhBMP-4 to perform Protein Expression Eukaryotic cells - CHO BMP-4

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using pIEX-5-6His-mMBP-UMODpXR to perform Protein Expression Eukaryotic cells - HEK293 mMBP

Get tips on using p1.2-Hygro-FSH-B-chain to perform Protein Expression Eukaryotic cells - CHO FSH

Get tips on using NucleoSpin® RNA/Protein to perform Protein isolation Mammalian cells - Human eutopic endometrial stromal cells

Get tips on using PageRuler™ Unstained Protein Ladder to perform Protein Ladder Unstained

Get tips on using IRIS9 Plus Prestained Protein Ladder to perform Protein Ladder Prestained