ChIP H3K27me3 Mouse Canine

- Found 2520 results

Get tips on using Histone H3K4me3 antibody (mAb) to perform ChIP Anti-bodies H3K4me3

Products Active Motif Histone H3K4me3 antibody (mAb)

Get tips on using Mouse CRP / C Reactive Protein / PTX1 PicoKine™ ELISA Kit to perform ELISA Mouse - C-Reactive Protein/CRP

Products BosterBio Mouse CRP / C Reactive Protein / PTX1 PicoKine™ ELISA Kit

Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) have been greatly used for studies on embryonic development and cell differentiation.iPSCs provide a stable source for either self-renewal or differentiation into suitable cells when cultured in a particular environment. Pluripotent cell culture was originally started by deriving cells from inner cell mass (ICM) from pre-implanted blastocysts, these were called embryonic stem cells. These cells after isolation can be grown on traditional extracellular matrices (like mouse embryonic fibroblasts, MEFs) or feeder-free culture systems. DMEM/F12 has been the most commonly used basal media in the culture of pluripotent cells. These cells are cultured at normal atmospheric oxygen levels, 21%, however, some studies have proposed that 4% oxygen tension may be better for hESC growth. Higher D-glucose concentration (4.2g/l) and osmolarity (320mOsm) that mimics the natural environment of embryonic tissue are optimal for the growth of hESCs. Supplements like N2 and/or B-27, in the presence of growth factors like bFGF, have been shown to increase pluripotency of these cells. bFGF, FGF2 and other ligands of receptor tyrosine kinases like IGF are also required or maintain self-renewal ability of these cells. TGF𝛃1, by its activation of SMAD2/3 signalling, also represses differentiation of iPSCs. Other compounds like ROCK inhibitors reduce blebbing and apoptosis in these cells to maintain their clonogenicity. However, an inhibitor for LIF (leukaemia inhibitory factor, which is one of the pluripotent genes) has an opposing effect. Therefore, it is important to understand the culture conditions and media composition that affect downstream signalling in hESCs or iPSCs that may lead to their differentiation.

Cell culture media Stem cell culture media Mouse myoblasts cells

Get tips on using VENTANA anti-MLH1 (M1) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MLH1

Products Roche Lifesciences VENTANA anti-MLH1 (M1) Mouse Monoclonal Primary Antibody

Get tips on using Human/Mouse/Rat Activin A Quantikine ELISA Kit to perform ELISA Rat - Activin

Products R&D Systems Human/Mouse/Rat Activin A Quantikine ELISA Kit

RNA RNA isolation / purification Tissue Mouse Vagina

Get tips on using CD273 (PD-L2) Antibody, anti-mouse, PerCP-Vio® 700 to perform Flow cytometry Anti-bodies Mouse - CD273/PD-L2

Products Miltenyibiotec CD273 (PD-L2) Antibody, anti-mouse, PerCP-Vio® 700

Get tips on using Monoclonal Anti-γ-Tubulin antibody produced in mouse to perform Western blotting tubulin gamma

Products Sigma-Aldrich Monoclonal Anti-γ-Tubulin antibody produced in mouse

Get tips on using Monoclonal Anti-Caveolin-1 antibody produced in mouse to perform Western blotting Caveolin-1

Products Sigma-Aldrich Monoclonal Anti-Caveolin-1 antibody produced in mouse

Get tips on using BRCA2 rtu elisa kit :: Mouse Breast Cancer Susceptibility Protein 2 (BRCA2) RTU ELISA Kit to perform ELISA Mouse - BRCA2

Products MyBioSource.com BRCA2 rtu elisa kit :: Mouse Breast Cancer Susceptibility Protein 2 (BRCA2) RTU ELISA Kit

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms