siRNA / miRNA gene silencing Rat RMC-1

Get tips on using Hs_PTEN_6 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HCT-116 PTEN1

Get tips on using Hs_DNMT3B_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HCT-116 DNMT3B

Get tips on using Hs_RELB_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HT-1080 RELB

Get tips on using Hs_RELA_3 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HT-1080 RELA

Get tips on using Hs_IFIH1_7 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HT-1080 IFIH1

Get tips on using Hs_DDX58_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HT-1080 DDX58

Get tips on using Hs_TLR3_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HT-1080 TLR3

RNAi or RNA interference is a common method to suppress gene expression in vitro/in vivo by utilizing the inherent microRNA machinery, without introducing a total gene knockout. miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with the desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid-mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time-consuming, but provide a more permanent expression of RNAi in the cells and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines.

Get tips on using Rab 7 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B RAB7A

Get tips on using Rab 5C siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B RAB5C