rna-isolation-purification-cells-primary-mouse-ventricles

Get tips on using FastDigest RsaI to perform Restriction Enzymes RsaI / AfaI

Get tips on using RsaI R6371 to perform Restriction Enzymes RsaI / AfaI

Get tips on using RsaI NEB#R0167 to perform Restriction Enzymes RsaI / AfaI

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using AfaI (RsaI) restriction enzyme to perform Restriction Enzymes RsaI / AfaI

Get tips on using RsaI (10 U/µL) to perform Restriction Enzymes RsaI / AfaI

Get tips on using RCAS1 siRNA (h) to perform siRNA / miRNA gene silencing Human - ES2 RCAS1

Get tips on using Human C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Human - C-Reactive Protein/CRP