Protein expression and purification Insect cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using pEGFP-INHα to perform Protein Expression Eukaryotic cells - BHK cells INHα

Get tips on using EMBacY#5 to perform Protein Expression Eukaryotic cells - Hi5 Soluble G protein (Hendra Virus)

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using pTip-QC2-gi_21218674 to perform Protein Expression Prokaryotic cells - R. erythropolis putative DNA-binding protein

Get tips on using pJAP2 to perform Protein Expression Eukaryotic cells - HEK293 AT1R

Get tips on using pJAP34 to perform Protein Expression Eukaryotic cells - HEK293 A1R

Get tips on using pJAP37 to perform Protein Expression Eukaryotic cells - HEK293 A1R

Get tips on using PL_MmXbp1s to perform Protein Expression Eukaryotic cells - CHO Xbp1s