Protein expression and purification Insect cells S2

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RNA isolation / purification Cells - primary porcine primary airway epithelial cell Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary porcine primary airway epithelial cell
RNA isolation / purification Cells - primary rat dorsal root ganglion neurons Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary rat dorsal root ganglion neurons
RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons Experiment

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary mouse dorsal root ganglion neurons
pEGFP-INHα Product

Get tips on using pEGFP-INHα to perform Protein Expression Eukaryotic cells - BHK cells INHα

Products Yaling Gu, Agriculture College, Ningxia University, Yinchuan, Ch pEGFP-INHα
EMBacY#5 Product

Get tips on using EMBacY#5 to perform Protein Expression Eukaryotic cells - Hi5 Soluble G protein (Hendra Virus)

Products Sabine Suppmann, Max-Planck Institute of Biochemistry EMBacY#5
RNA isolation / purification Cells - primary mouse morula cells Experiment

RNA RNA isolation / purification Cells primary mouse morula cells
DNA isolation / purification Cells - Immortalized cell lines Lymphoblastoid cell lines Experiment

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Immortalized cell lines Lymphoblastoid cell lines
pTip-QC2-gi_21218674 Product

Get tips on using pTip-QC2-gi_21218674 to perform Protein Expression Prokaryotic cells - R. erythropolis putative DNA-binding protein

Products Tomoshi Kameda, Artificial Intelligence Research Center, Nationa pTip-QC2-gi_21218674
pJAP2 Product

Get tips on using pJAP2 to perform Protein Expression Eukaryotic cells - HEK293 AT1R

Products Christopher G. Tate, MRC Laboratory of Molecular Biology, Cambri pJAP2
pJAP34 Product

Get tips on using pJAP34 to perform Protein Expression Eukaryotic cells - HEK293 A1R

Products Christopher G. Tate, MRC Laboratory of Molecular Biology, Cambri pJAP34

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