The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.
Get tips on using Silencer select_FGA siRNA to perform siRNA / miRNA gene silencing Human - HepG2 FGA
Get tips on using Silencer select_FGB siRNA to perform siRNA / miRNA gene silencing Human - HepG2 FGB
Get tips on using Silencer select_FGG siRNA to perform siRNA / miRNA gene silencing Human - HepG2 FGG
Get tips on using siGENOME PCNT siRNA to perform siRNA / miRNA gene silencing Human - HEK293 PCNT
Get tips on using siGENOME TRIM43 siRNA to perform siRNA / miRNA gene silencing Human - HEK293 TRIM43
Get tips on using CDK5RAP1 siRNA (h) to perform siRNA / miRNA gene silencing Human - A375 CDK5RAP1
Get tips on using VEGF siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC VEGFA
Get tips on using RCAS1 siRNA (h) to perform siRNA / miRNA gene silencing Human - ES2 RCAS1
Get tips on using HtrA2 siRNA (h) to perform siRNA / miRNA gene silencing Human - A549 HtrA2
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