siRNA / miRNA gene silencing Human HNSCC cell line

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DNA insert using CRISPR Discussion

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

Protein Expression Eukaryotic cells - CHO BMP-4 Experiment

Proteins Protein Expression Eukaryotic cells CHO BMP-4

Protein expression and purification Insect cells - Sf9 Drosha Experiment

Proteins Protein expression and purification Insect cells Sf9 Drosha

Protein expression and purification Insect cells - Hi5 TYR Experiment

Proteins Protein expression and purification Insect cells Hi5 TYR

Protein expression and purification Insect cells - S2 HER2 Experiment

Proteins Protein expression and purification Insect cells S2 HER2

Protein expression and purification Mammalian cells - HeLa ChaC1 Experiment

Proteins Protein expression and purification Mammalian cells HeLa ChaC1

Protein Expression Prokaryotic cells - E. coli Integrin αV Experiment

Proteins Protein Expression Prokaryotic cells E. coli Integrin αV

pEGFP-INHα Product

Get tips on using pEGFP-INHα to perform Protein Expression Eukaryotic cells - BHK cells INHα

Products Yaling Gu, Agriculture College, Ningxia University, Yinchuan, Ch pEGFP-INHα

TRIzol Reagent Product

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized HSG cells

Products Thermo Fisher Scientific TRIzol Reagent

CelLytic™ M Product

Get tips on using CelLytic™ M to perform Protein isolation Bacteria - Staphylococcus aureus

Products Sigma-Aldrich CelLytic™ M

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