Immunohistochemistry Anti-rabbit IgG Donkey

Get tips on using Mouse/Rat TrkC Antibody to perform Immunohistochemistry Mouse - TrkC

Get tips on using SQSTM1/p62 Antibody #5114 to perform Immunohistochemistry Mouse - p62

Get tips on using p62 (human) polyclonal antibody to perform Immunohistochemistry Mouse - p62

Get tips on using Anti-phospho-Histone H2A.X (Ser139) Antibody to perform TissueFAxs phospho-Histone H2A.X (Ser139) - Mouse Human

Get tips on using Anti-phospho-Histone H2A.X (Ser139) Antibody to perform Immunofluorscence  phospho-Histone H2A.X (Ser139) - Mouse Human

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using Human FGF-10 Antibody to perform Immunohistochemistry Human - FGF-10

Get tips on using TTF1 Monoclonal Antibody (2F4D8) to perform Immunohistochemistry Human - TTF-1

Get tips on using Mouse Osteopontin/OPN Antibody to perform Immunohistochemistry Mouse - Spp1/OPN

Get tips on using Anti-p62 (SQSTM1) (Human) pAb to perform Autophagy assay cell type - A549