siRNA / miRNA gene silencing Human HNSCC cell line Eph receptor B4

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DNA isolation / purification Cells - Primary cells HUVEC Experiment

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

DNA DNA isolation / purification Cells Primary cells HUVEC

What DNA isolation kit would work for insect samples? Discussion

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

Discussions What DNA isolation kit would work for insect samples?

DNA insert using CRISPR Discussion

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

Protein Expression Eukaryotic cells - CHO BMP-4 Experiment

Proteins Protein Expression Eukaryotic cells CHO BMP-4

Protein expression and purification Insect cells - Sf9 Drosha Experiment

Proteins Protein expression and purification Insect cells Sf9 Drosha

Protein expression and purification Insect cells - Hi5 TYR Experiment

Proteins Protein expression and purification Insect cells Hi5 TYR

Protein expression and purification Insect cells - S2 HER2 Experiment

Proteins Protein expression and purification Insect cells S2 HER2

Protein expression and purification Mammalian cells - HeLa ChaC1 Experiment

Proteins Protein expression and purification Mammalian cells HeLa ChaC1

Protein Expression Prokaryotic cells - E. coli Integrin αV Experiment

Proteins Protein Expression Prokaryotic cells E. coli Integrin αV

pEGFP-INHα Product

Get tips on using pEGFP-INHα to perform Protein Expression Eukaryotic cells - BHK cells INHα

Products Yaling Gu, Agriculture College, Ningxia University, Yinchuan, Ch pEGFP-INHα

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