siRNA / miRNA gene silencing Human ES2(ovarian cancer cell line)

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Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

Discussions What DNA isolation kit would work for insect samples?

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

Proteins Protein Expression Eukaryotic cells CHO BMP-4

Proteins Protein expression and purification Insect cells Sf9 Drosha

Proteins Protein expression and purification Insect cells Hi5 TYR

Proteins Protein expression and purification Insect cells S2 HER2

Proteins Protein expression and purification Mammalian cells HeLa ChaC1

Proteins Protein Expression Prokaryotic cells E. coli Integrin αV

Get tips on using pEGFP-INHα to perform Protein Expression Eukaryotic cells - BHK cells INHα

Products Yaling Gu, Agriculture College, Ningxia University, Yinchuan, Ch pEGFP-INHα

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized HSG cells

Products Thermo Fisher Scientific TRIzol Reagent

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