Angiogenesis assay

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis HUVEC

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis HeLa

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis MCF7

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - Raw 264.7

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - 3T3-L1

Get tips on using Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells to perform Live / Dead assay bacteria - Salmonella enterica

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - rat plasma

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis HuH-7

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis PANC-1