Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - BEAS-2B human bronchial epithelial cell line
Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Cell cytotoxicity / Proliferation assay cell type - THP-1
Get tips on using EpiCult™-B Mouse Medium Kit to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres
Get tips on using Cyclin E1 (HE12) Mouse mAb #4129 to perform Western blotting Cyclin E
Get tips on using Autophagy Assay Kit to perform Autophagy assay cell type - Beas-2B
Get tips on using IntestiCult™ Organoid Growth Medium (Mouse) to perform 3D Cell Culture Media Mouse gastric cancer organoids
Get tips on using IntestiCult™ Organoid Growth Medium (Mouse) to perform 3D Cell Culture Media Mouse small intestinal organoids
A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.
Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay .
Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Mouse - NIH 3T3
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