siRNA / RNAi /miRNA transfection Human Cells HESC

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Ovarian cancer cell line ES-2

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Colon cancer cell line LS-174T

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Colon cancer cell line DLD-1

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Colon cancer cell line NCI-H508

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Colon cancer cell line HCT-15

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Colon cancer cell line HCT-116

Get tips on using Corning® BioCoat™ Tumor Invasion 24-well Plate to perform Cell migration / Invasion cell type - SaOS-2

Get tips on using Culture-Insert 4 Well in µ-Dish 35 mm, high to perform Cell migration / Invasion cell type - MCF7

Get tips on using Click-iT™ EdU Alexa Fluor™ 555 Imaging Kit to perform Cell cytotoxicity / Proliferation assay cell type - PC-3

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.