Get tips on using MethylFlash™ Methylated DNA Quantification Kit to perform DNA quantification Brain tissue
Get tips on using RNeasy Fibrous Tissue Mini Kit to perform RNA isolation / purification Tissue - Human Teeth / Jaw
Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - mouse adipose tissue
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using DNeasy Blood and Tissue Kit (250) to perform DNA isolation / purification Cells - Immortalized cell lines Loucy
Get tips on using AllPrep DNA/RNA FFPE Kit to perform DNA isolation / purification Tissue - FFPE samples
Get tips on using MagAttract PowerSoil DNA KF Kit to perform DNA isolation / purification Tissue - fecal sample
Get tips on using AllPrep DNA/RNA Mini Kit to perform DNA isolation / purification Tissue - fecal sample
Get tips on using QIAamp 96 DNA Blood Kit to perform DNA isolation / purification Tissue - blood / plasma
Get tips on using QIAamp DNA Blood Midi Kit to perform DNA isolation / purification Tissue - blood / plasma
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