RNA isolation / purification Yeast

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Mouse Muscle

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Mouse Liver

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - immortalized C6

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - immortalized C2C12

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - immortalized U937

Get tips on using SV Total RNA Isolation System to perform

Contamination can affect cell characteristics, i.e., growth, metabolism, and morphology leading to unreliable and erroneous experimental data. Depending on the source of contaminants, one can detect contamination by using a light microscope, gram stain, isothermal amplification, or PCR. Bacteria and fungi can usually be identified by optical microscopy. Mycoplasma in cell cultures cannot be detected visually. Hence, these microbes can go unnoticed for long periods and are determined using dedicated assays. Early and rapid identification of contaminants is vital to detect, handle and prevent contamination for good cell-culture practices. However, detection and identification can be challenging and tricky based on usual visual identifications. Hence it is essential to use a standard contamination detection kit to detect and maintain best practices.

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - rat pancreas tissue

Get tips on using LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry to perform Live / Dead assay yeast - Urediniospore