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siRNA / miRNA gene silencing

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RNAi or RNA interference is a common method to suppress gene expression in vitro/in vivo by utilizing the inherent microRNA machinery, without introducing a total gene knockout. miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid-mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time-consuming, but provide a more permanent expression of RNAi in the cells and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines.

RNA siRNA / RNAi /miRNA transfection Rat IEC-6 Cationic lipid based

Get tips on using ON-TARGETplus Rat Dlc1 (58834) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Dlc1

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Get tips on using ON-TARGETplus Rat Rhoa (117273) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Rhoa

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Get tips on using ON-TARGETplus Rat Rhoc (295342) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 RhoC

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Get tips on using ON-TARGETplus Rat Rhog (308875) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 RhoG

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Get tips on using ON-TARGETplus Rat Trio (310192) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Trio

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Get tips on using ON-TARGETplus Rat Pak1 (29431) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - MTLn3 Pak1

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Get tips on using ON-TARGETplus Rat Nos3 (24600) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - NPC Nos3

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Get tips on using ON-TARGETplus Rat Klf15 (85497) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - NRCM Klf15

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Get tips on using ON-TARGETplus Rat Atf4 (79255) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Rat - PC12 Atf4

Products Horizon Discovery Ltd. ON-TARGETplus Rat Atf4 (79255) siRNA - SMARTpool

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