siRNA / miRNA gene silencing Human HNSCC cell line

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Get tips on using SignalSilence® IκBα siRNA to perform siRNA / miRNA gene silencing Human - Caco-2 IκBα

Products Cell Signaling Technology SignalSilence® IκBα siRNA

Get tips on using ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 Rab Coupling Protein (RCP)

Products Horizon Discovery Ltd. ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Discussions siRNA/RNAi/miRNA transfection human

Get tips on using Stealth siRNA(h)_GATA1 to perform siRNA / miRNA gene silencing Human - LAD2 GATA1

Products Thermo Fisher Scientific Stealth siRNA(h)_GATA1

Get tips on using Silencer® Select_HDAC8 siRNA to perform siRNA / miRNA gene silencing Human - UCC HDAC8

Products Thermo Fisher Scientific Silencer® Select_HDAC8 siRNA

Get tips on using Silencer® Select_SPRY2 siRNA to perform siRNA / miRNA gene silencing Human - RMS SPRY2

Products Thermo Fisher Scientific Silencer® Select_SPRY2 siRNA

Get tips on using CREB-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC CREB

Products Santa Cruz Biotechnology CREB-1 siRNA (h)

Get tips on using MEK-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - NHLF MEK1

Products Santa Cruz Biotechnology MEK-1 siRNA (h)

Get tips on using ERK 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - NHLF ERK1

Products Santa Cruz Biotechnology ERK 1 siRNA (h)

Get tips on using ENX-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - HaCaT EZH2

Products Santa Cruz Biotechnology ENX-1 siRNA (h)

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