The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.
Get tips on using Gβ 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D GNB1
Get tips on using Stealth siRNA™ NOTCH2 to perform siRNA / miRNA gene silencing Human - THP-1 NOTCH2
Get tips on using Stealth siRNA™ NOTCH1 to perform siRNA / miRNA gene silencing Human - THP-1 NOTCH1
Get tips on using caveolin-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B CAV1
Get tips on using Flotillin-2 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B FLOT2
Get tips on using Rab 7 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B RAB7A
Get tips on using Rab 5C siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B RAB5C
Get tips on using AMPKα1/2 siRNA (h) to perform siRNA / miRNA gene silencing Human - HepG2 AMPKα1/α2
Get tips on using GLI-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - NHLF GLI-1
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