siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

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Get tips on using NucleoSpin® Gel and PCR Clean-up to perform DNA gel extraction / PCR product purification Product size > 15Kb

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Get tips on using Illustra GFX PCR DNA and Gel Band Purification kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

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Get tips on using PureLink™ Quick Gel Extraction Kit and PCR Purification Combo Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

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I intend to use iScript cDNA Synthesis Kit in order to synthesize cDNA for qPCR. I have confirmed that my RNA is pure however, according to my extraction protocol I have suspended the RNA in TE buffer containing 1mM EDTA. Will the presence of EDTA have an effect on cDNA synthesis?

Discussions Will presence of EDTA effect cDNA synthesis

Get tips on using Desmin Antibody to perform Immunohistochemistry Desmin - Goatt Mouse -NA-

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Get tips on using QIAprep Spin Miniprep Kit to perform Plasmid Isolation Neisseria gonorrhoeae

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Get tips on using RNeasy Plant Mini Kit to perform RNA isolation / purification Yeast - Ashbya gossypii

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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Neisseria gonorrhoeae

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Get tips on using Anti-Type I Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-

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Get tips on using RiboPure™ RNA Purification Kit, yeast to perform RNA isolation / purification Yeast - Ashbya gossypii

Products Thermo Fisher Scientific RiboPure™ RNA Purification Kit, yeast

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