RNA sequencing

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized A-172

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - mouse lung tissue

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - mouse brain tissue

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - mouse adipose tissue

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Tissue - mouse liver tissue

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - human brain tissue

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human melanocytes

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - human liver tissue

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.