Immunohistochemistry 53BP2 phospho (ser-25) Rabbit IgG Human

Get tips on using Human IGF1 ELISA Kit (ab100545) to perform ELISA Human - IGF-I

Get tips on using Anti-acetyl-Histone H4 Antibody to perform ChIP acH4 - Rabbit Sheep YFP Tag

Get tips on using Goat Anti-Type III Collagen-BIOT to perform Immunohistochemistry Collagen Type III - Goat Mouse Biotin

Get tips on using Recombinant Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) to perform Immunohistochemistry Mouse - β-Catenin

Get tips on using Recombinant Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063) to perform Immunohistochemistry Mouse - ERα

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Human IGF-I/IGF-1 Quantikine ELISA Kit to perform ELISA Human - IGF-I

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rabbit aortic smooth muscle cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rabbit aortic endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rabbit skeletal muscle cells