siRNA / RNAi /miRNA transfection Human Cells Cal 27 cells

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using SuperScript IV VILO Master Mix to perform cDNA synthesis Cell lines

Get tips on using Annexin V / 7-AAD to perform Apoptosis assay cell type - MCF-7

Get tips on using Annexin V-FITC Kit to perform Apoptosis assay cell type - OVCAR-3

Get tips on using bisBenzimide H 33342 trihydrochloride to perform Apoptosis assay cell type - OVCAR-3

Get tips on using Acid phosphatase Assay Kit to perform Acid phosphatase assay cell type - OV2008

Get tips on using SuperScript® II Reverse Transcriptase to perform cDNA synthesis Cell lines

Get tips on using RevertAid RT Reverse Transcription Kit to perform cDNA synthesis Cell lines

Get tips on using CAG-Cas9 to perform CRISPR Mouse - Deletion Neuro 2a Rab38

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.