TissueFAxs 53BP1 [H-300] Rabbit

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab9050) to perform ChIP Anti-bodies H3K36me3

Get tips on using Anti-Histone H3 (mono methyl K36) antibody - ChIP Grade (ab9048) to perform ChIP Anti-bodies H3K36me1

Get tips on using Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (ab8898) to perform ChIP Anti-bodies H3K9me3

Get tips on using Anti-Histone H3 (di methyl K27) antibody - ChIP Grade (ab24684) to perform ChIP Anti-bodies H3K27me2

Get tips on using Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580) to perform ChIP Anti-bodies H3K4me3

Get tips on using Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (ab7766) to perform ChIP Anti-bodies H3K4me2

Get tips on using Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895 to perform ChIP Anti-bodies H3K4me1

Get tips on using Q5® Hot Start High-Fidelity 2X Master Mix to perform PCR Hot start PCR - Mammalian DNA

Get tips on using Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade to perform ChIP H3K27me3 - Sheep Rat YFP Tag

Stem cells have the unique ability to self-renew or differentiate themselves into various cell types in response to appropriate signals. These cells are especially important for tissue repair, regeneration, replacement, or in the case of hematopoietic stem cells (HSCs) to differentiate into various myeloid populations. Appropriate signals refer to the growth factor supplements or cytokines that mediate differentiation of various stem cells into the required differentiated form. For instance, HSCs can be differentiated into dendritic cells (with IL-4 and GM-CSF), macrophages (with m-CSF) and MDSCs (with IL-6 and GM-CSF). Human pluripotent stem cells (hPSCs) and induced pluripotent stem cells (iPSCs) can be first cultured in neural differentiation media (GSK3𝛃-i, TGF𝛃-i, AMPK-i, hLIF) to form neural rosettes, which can be differentiated into neural or glial progenitors (finally differentiated into oligodendrocytes). Neural progenitors can be finally differentiated into glutaminergic (dibytyryl cAMP, ascorbic acid) and dopaminergic (SHH, FGF-8, BDNF, GDNF, TGF-𝛃3) neurons. Thus, it is important to first identify the self-renewing cell line: its source and its final differentiation state, followed by the supplements and cytokines required for the differentiation, and final use. Timelines are another thing that is considered. For instance, it takes 7-10 days to form neural rosettes from iPSCs and 3 days to differentiate neural progenitors to neurons. Finally, the stability for stem cell culture media varies. It is advised to make fresh media every time when differentiating HSCs to myeloid populations, whereas neural differentiation media may remain stable for two weeks when stored in dark between 2-8C.