Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Bronchi
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Breast
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Human Bone
Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse kidney tissue
Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse liver tissue
Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse adipose tissue
DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Rat Thyroid gland
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment