Protein Expression Prokaryotic cells C. glutamicum

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Get tips on using Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate to perform Flowcytometry Secondary Antibody - Goat Rabbit Alexa Fluor 488

Products Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR
MLM3636 Product

Get tips on using MLM3636 to perform CRISPR Human - Activation VEGFA

Products Addgene MLM3636
hCas9 Product

Get tips on using hCas9 to perform CRISPR Human - Deletion Hsp90α

Products Addgene hCas9
hCas9 Product

Get tips on using hCas9 to perform CRISPR Mouse - Deletion Dck

Products Addgene hCas9

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression

Products Addgene lentiCRISPR v2

Get tips on using MicroRNA isolation kit to perform CRISPR

Products A&A Biotechnology MicroRNA isolation kit
hCas9 Product

Get tips on using hCas9 to perform CRISPR Mouse - Deletion B117P Dck

Products Addgene hCas9
pCas9_GFP Product

Get tips on using pCas9_GFP to perform CRISPR Mouse - Deletion ANS4 Rosa26

Products Addgene pCas9_GFP
X-AC9 Product

Get tips on using X-AC9 to perform Immunohistochemistry Mouse - Col X

Products Developmental Studies Hybridoma Bank X-AC9

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