Protein Expression Prokaryotic cells A. cellulolyticus

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Get tips on using MACSprep™ HLA B/T Cell Isolation Kit, human to perform Cell Isolation HLA B/T

Products Miltenyibiotec MACSprep™ HLA B/T Cell Isolation Kit, human

Get tips on using X-VIVOTM 10 Serum-free Hematopoietic Cell Medium to perform 3D Cell Culture Media Mouse thymic organoids

Products Lonza X-VIVOTM 10 Serum-free Hematopoietic Cell Medium

Get tips on using SmGMTM- 2 Smooth Muscle Cell Growth Medium -2 BulletKitTM to perform Mammalian cell culture media HCASMC

Products Lonza SmGMTM- 2 Smooth Muscle Cell Growth Medium -2 BulletKitTM

Get tips on using SmGMTM- 2 Smooth Muscle Cell Growth Medium -2 BulletKitTM to perform Mammalian cell culture media HCtASMC

Products Lonza SmGMTM- 2 Smooth Muscle Cell Growth Medium -2 BulletKitTM

Get tips on using EBMTM Endothelial Cell Growth Basal Medium, 500 mL to perform 3D Cell Culture Media Human blood-brain barrier organoid

Products Lonza EBMTM Endothelial Cell Growth Basal Medium, 500 mL

Get tips on using EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM to perform Stem cell culture media hPericytes

Products Lonza EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM

Get tips on using EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM to perform Stem cell Differentiation media hMSCs differentiation into pericytes

Products Lonza EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM

I intend to use iScript cDNA Synthesis Kit in order to synthesize cDNA for qPCR. I have confirmed that my RNA is pure however, according to my extraction protocol I have suspended the RNA in TE buffer containing 1mM EDTA. Will the presence of EDTA have an effect on cDNA synthesis?

Discussions Will presence of EDTA effect cDNA synthesis

RNA RNA isolation / purification Supernatant from cell cultures

Get tips on using Cell Proliferation Kit I (MTT) to perform

Products Sigma-Aldrich Cell Proliferation Kit I (MTT)

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