siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

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Get tips on using Strep-Tactin Superflow Plus (10 ml) to perform Protein tag Purification of Strep-tagged proteins

Products Qiagen Strep-Tactin Superflow Plus (10 ml)

Get tips on using MaXtract High Density (100 x 15 ml) to perform DNA isolation / purification Tissue - small intestine

Products Qiagen MaXtract High Density (100 x 15 ml)

Get tips on using Ni-NTA Magnetic Agarose Beads (6 x 1 ml) to perform Protein tag Purification of His-tagged proteins

Products Qiagen Ni-NTA Magnetic Agarose Beads (6 x 1 ml)

Get tips on using Gyros IncSupplier Diversity Partner REXXIP HN BUFFER 25 ML PER VI DFS Item to perform Protein isolation Bacteria - Borrelia burgdorferi

Products Fisher Scientific Gyros IncSupplier Diversity Partner REXXIP HN BUFFER 25 ML PER VI DFS Item

I have extracted RNA from brain tissue but my RNA concentrations are as low as 5ng/ml with my highest being around 80ng/ml. Will I be able to perform cDNA conversion with concentrations as low as these?

Discussions cDNA conversion with low concentrations

Get tips on using FastDigest SchI to perform Restriction Enzymes SchI / MlyI

Products Thermo Fisher Scientific FastDigest SchI

Get tips on using MscI NEB#R0534 to perform Restriction Enzymes MlsI / MscI

Products New England BioLabs MscI NEB#R0534

Get tips on using Anti-CTCF Antibody to perform ChIP Anti-bodies CTCF

Products Merck Millipore Anti-CTCF Antibody

Get tips on using APC anti-mouse CD103 Antibody to perform Flow cytometry Anti-bodies Mouse - CD103

Products BioLegend APC anti-mouse CD103 Antibody

Get tips on using Purified anti-mouse CD68 Antibody to perform Flow cytometry Anti-bodies Mouse - CD68

Products BioLegend Purified anti-mouse CD68 Antibody

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