siRNA / RNAi /miRNA transfection Mouse Glomerular Mesangial cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using RAB8A siRNA to perform siRNA / miRNA gene silencing Human - HEK293 Rab8a

Get tips on using KRAS siRNA to perform siRNA / miRNA gene silencing Human - A431 KRAS

Get tips on using STEAP1 siRNA to perform siRNA / miRNA gene silencing Human - LNCap STEAP1

Get tips on using siRNA s2748 to perform siRNA / miRNA gene silencing Human - HeLa Cdc20

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - RWPE SKIL

Get tips on using siRNA SKIL to perform siRNA / miRNA gene silencing Human - LNCap SKIL

Get tips on using siRNA PIN1 to perform siRNA / miRNA gene silencing Human - LNCap PIN1

Get tips on using siRNA AURKA to perform siRNA / miRNA gene silencing Human - H1299 AURKA