siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

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Illustra GFX PCR DNA and Gel Band Purification kit Product

Get tips on using Illustra GFX PCR DNA and Gel Band Purification kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Products Fisher Scientific Illustra GFX PCR DNA and Gel Band Purification kit

PureLink™ Quick Gel Extraction Kit and PCR Purification Combo Kit Product

Get tips on using PureLink™ Quick Gel Extraction Kit and PCR Purification Combo Kit to perform DNA gel extraction / PCR product purification Product size < 15Kb

Products Thermo Fisher Scientific PureLink™ Quick Gel Extraction Kit and PCR Purification Combo Kit

Will presence of EDTA effect cDNA synthesis Discussion

I intend to use iScript cDNA Synthesis Kit in order to synthesize cDNA for qPCR. I have confirmed that my RNA is pure however, according to my extraction protocol I have suspended the RNA in TE buffer containing 1mM EDTA. Will the presence of EDTA have an effect on cDNA synthesis?

Discussions Will presence of EDTA effect cDNA synthesis

CellTiter-Glo® Luminescent Cell Viability Assay Product

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Cell cytotoxicity / Proliferation assay cell type - HT-22

Products Promega CellTiter-Glo® Luminescent Cell Viability Assay

CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) Product

Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - A549