Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - rat kidney tissue
Get tips on using GenCatch™ Total RNA Extraction Kit to perform RNA isolation / purification Tissue - rat liver tissue
Get tips on using AllPrep DNA/RNA Micro Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary human umbilical vein endothelial cells
Get tips on using ISOLATE II RNA Micro Kit to perform RNA isolation / purification Cells - primary human carotid artery endothelial cells
Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells
Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized SKBR3, MDA-MB231 and MCF7
Get tips on using E.Z.N.A.® Total RNA Kit I to perform RNA isolation / purification Cells - primary rat cardiac fibroblasts
The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.
The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads could be the best alternative.
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