Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP)

Get tips on using Purified Hamster Anti-Mouse TCR β Chain to perform Flow cytometry Anti-bodies Mouse - TCRbeta

Get tips on using Purified NA/LE Hamster Anti-Mouse CD40 to perform Flow cytometry Anti-bodies Mouse - CD40

Get tips on using C/EBP β siRNA (r) to perform siRNA / miRNA gene silencing Rat - Glial cells C/EBP‐β

Get tips on using Biotin Rat Anti-Mouse OX40 Ligand (CD252) to perform Flow cytometry Anti-bodies Mouse - CD252/OX40L

Get tips on using PE-CF594 Rat Anti-Mouse Siglec-F to perform Flow cytometry Anti-bodies Mouse - Siglec F

Get tips on using Anti-Beclin 1 (Human) pAb to perform Autophagy assay cell type - UMR-106

Get tips on using Anti-p62 (SQSTM1) (Human) pAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Anti-Beclin 1 (Human) pAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Cytokeratin 19 Antibody to perform Immunohistochemistry Cytokeratin 19 - Rabbit Mouse -NA-

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.