Stem cell culture media

Get tips on using Iscove's Modified Dulbecco's Medium (IMDM) (ATCC® 30-2005™) to perform Mammalian cell culture media KG1

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)

Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media Human breast cancer MCF-7 cells-Mammospheres

Get tips on using EpiCult™-B Mouse Medium Kit to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary breast cancer ephitelial cells-Mammospheres

Get tips on using DMEM with Glucose and L-Glutamine to perform 3D Cell Culture Media U87MG cells- glioblastoma spheres

Get tips on using DMEM/F-12, no phenol red to perform 3D Cell Culture Media BT-549 cells-Mammospheres

Get tips on using BrainPhys™ Without Phenol Red to perform Stem cell Differentiation media Differentiation of Human iPSCs into Basal Forebrain cholinergic neurons (BFCN)

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Mammalian cell culture media MDA-MB-231