siRNA / RNAi /miRNA transfection Human Cells Cal 27 cells

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - 3T3-L1

Get tips on using anti-p62 / SQSTM1 (C-terminus) guinea pig polyclonal, serum to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - rat plasma

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell Differentiation media iPSCs or hESCs differentiation into cerebellar neuroepithelium (NE)

Get tips on using OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) to perform ROS assay cell type - mouse dorsal skin tissue

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)

Get tips on using Vybrant™ Apoptosis Assay Kit #4, YO-PRO™-1/Propidium Iodide to perform Apoptosis assay cell type - MG-63

Get tips on using Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy to perform ROS assay cell type - MCF-7

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform Apoptosis assay cell type - BxPC-3