Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP)

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Anti-gamma H2A.X (phospho S139) antibody - ChIP Grade (ab2893) to perform ChIP Anti-bodies γH2AX

Get tips on using Monoclonal ANTI-FLAG® M2 antibody produced in mouse to perform ChIP Anti-bodies FLAG

Get tips on using Recombinant Anti-Histone H3.3 antibody [EPR17899] - ChIP Grade (ab176840) to perform ChIP Anti-bodies H3.3

Get tips on using Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) to perform ChIP Anti-bodies H3K27me3

Get tips on using Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (ab4729) to perform ChIP Anti-bodies H3K27ac

Get tips on using PE Mouse Anti-Human CD31 Clone L133.1 to perform Flow cytometry Anti-bodies Human - CD31/PECAM-1

Get tips on using Anti-ATP5A antibody [15H4C4] - Mitochondrial Marker (ab14748) to perform Western blotting ATP5A

Get tips on using Monoclonal Anti-TBP antibody produced in mouse to perform Western blotting TBP

Get tips on using Purified anti-mouse/rat/human FOXP3 Antibody to perform Western blotting FOXP3