Immunohistochemistry Wilms Tumor 1 (WT1) Rabbit

Get tips on using Anti-Villin antibody [3E5G11] - N-terminal (ab201989) to perform Immunohistochemistry Human - Villin

Get tips on using Anti-Glutamine Synthetase Antibody, clone GS-6 to perform Immunohistochemistry Rat - GS

Get tips on using GATA-4 Antibody (G-4): sc-25310 to perform Immunohistochemistry Mouse - Gata4

Get tips on using Monoclonal Mouse Anti-Bromodeoxyuridine (Concentrate) Clone Bu20a to perform Immunohistochemistry Mouse - BrdU

Get tips on using Smooth Muscle Actin Antibody (B4): sc-53142 to perform Immunohistochemistry Mouse - SMA

Get tips on using Anti-alpha smooth muscle Actin antibody (ab5694) to perform Immunohistochemistry Mouse - SMA

Get tips on using Anti-CD133 antibody - Stem Cell Marker (ab19898) to perform Immunohistochemistry Mouse - CD133

Get tips on using Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker to perform Immunohistochemistry Mouse - p62

Get tips on using Recombinant Anti-Lysozyme antibody [EPR2994(2)] (ab108508) to perform Immunohistochemistry Mouse - Lysozyme

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.