Protein expression and purification Tissue

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - mouse lung tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - mouse brain tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - mouse ear tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - mouse bone tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - mouse aorta tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - mouse liver tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - mouse muscle tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human breast tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human lung tissue