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DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Wilms' Tumor 1 (WT1) Protein, Clone 6F-H2 to perform Immunohistochemistry Wilms Tumor 1 (WT1) - Rabbit Mouse -NA-

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

Get tips on using AllPrep Bacterial DNA/RNA/Protein Kit (50) to perform DNA isolation / purification Bacteria - Gram negative Massilia sp

Get tips on using RNeasy DSP FFPE Kit to perform RNA isolation / purification Tissue - Human FFPE tissue

Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using Absolutely RNA FFPE Kit to perform RNA isolation / purification Tissue - rat kidney tissue

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - human placental tissue

Get tips on using RNeasy Plus Micro Kit to perform RNA isolation / purification Tissue - human liver tissue

Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - human kidney tissue