Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Lymph node
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Rat Bone marrow
Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Rat Hippocampus
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Thyroid gland
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Spinal cord
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using Total RNA Kit, peqGOLD to perform RNA isolation / purification Tissue - Human Stomach
Get tips on using NucleoSpin® RNA XS to perform RNA isolation / purification Tissue - Human Skin
Get tips on using NucleoSpin® RNA/Protein to perform RNA isolation / purification Tissue - Human Cornea
Get tips on using Gentra Puregene Mouse Tail Kit (4 g) to perform DNA isolation / purification Tissue - murine tail biopsies
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