rna-isolation-tissue

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse liver tissue

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse lung tissue

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - mouse aorta tissue

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - human placental tissue

Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Tissue - human brain tissue

Get tips on using Ambion™ RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE to perform RNA isolation / purification Tissue - Human Kidney

Get tips on using Qproteome FFPE Tissue Kit (20) to perform Protein isolation Tissue - Human tissue C-MFPE samples

Get tips on using TRIzol™ Max™ Bacterial RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.