rna-sequencing-mouse-3t3-l1

Get tips on using Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit to perform ELISA Mouse - RANK L

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Human/Mouse/Rat SOX2 Antibody to perform Western blotting SOX2

Get tips on using FoxP3 Antibody, anti-mouse, PE, REAfinity™ to perform Flow cytometry Anti-bodies Mouse - FOXP3

Get tips on using Human/Mouse/Rat/Canine ALCAM/CD166 Antibody to perform Immunohistochemistry Mouse - CD166 / ALCAM

Get tips on using Rat/Mouse Cytochrome c Quantikine ELISA Kit to perform ELISA Mouse - Cytochrome c

Get tips on using Purified anti-mouse TNF-α Antibody to perform Flow cytometry Anti-bodies Mouse - TNF-α

Get tips on using FITC anti-mouse CD95 (Fas) Antibody to perform Flow cytometry Anti-bodies Mouse - CD95/Fas

Get tips on using APC anti-mouse CD252 (OX40L) Antibody to perform Flow cytometry Anti-bodies Mouse - CD252/OX40L

Get tips on using siGENOME Mouse Amotl2 (56332) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - MS1 AmotL2