Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - THP 1
Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y
In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.
Get tips on using Anti-53BP1 (phospho S25) antibody, rabbit polyclonal to perform Immunohistochemistry 53BP2 phospho (ser-25) - Rabbit IgG Human -NA-
Get tips on using DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793 to perform ChIP Anti-bodies FLAG
Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HK-2 cells
Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Cell cytotoxicity / Proliferation assay cell type - K562
In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.
Get tips on using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 to perform Western blotting p21
Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19
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