ChIP H3K27me3 Rabbit Chicken

- Found 594 results

Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - THP 1

Products Cell Signaling Technology SQSTM1/p62 (D5E2) Rabbit mAb

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Products Cell Signaling Technology Beclin-1 (D40C5) Rabbit mAb

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Proteins ChIP Anti-bodies H3K36me3

Get tips on using Anti-53BP1 (phospho S25) antibody, rabbit polyclonal to perform Immunohistochemistry 53BP2 phospho (ser-25) - Rabbit IgG Human -NA-

Products Abcam Anti-53BP1 (phospho S25) antibody, rabbit polyclonal

Get tips on using DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793 to perform ChIP Anti-bodies FLAG

Products Cell Signaling Technology DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HK-2 cells

Products Cell Signaling Technology Beclin-1 (D40C5) Rabbit mAb

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Cell cytotoxicity / Proliferation assay cell type - K562

Products Cell Signaling Technology Beclin-1 (D40C5) Rabbit mAb

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Proteins ChIP Anti-bodies H3K9me3

Get tips on using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 to perform Western blotting p21

Products Cell Signaling Technology p21 Waf1/Cip1 (12D1) Rabbit mAb #2947

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19

Products Cell Signaling Technology Beclin-1 (D40C5) Rabbit mAb

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