ChIP acH3 Rat Sheep

- Found 2022 results

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Proteins ChIP Mouse Cardiac fibroblasts

Get tips on using OneDay ChIP kit to perform ChIP Human - Kupffer Cells

Products Diagenode OneDay ChIP kit

Get tips on using ChIP Kit (ab500) to perform ChIP Human - HUH-7

Products Abcam ChIP Kit (ab500)
EZ-ChIP™ Product

Get tips on using EZ-ChIP™ to perform ChIP Human - HUH-7

Products Merck Millipore EZ-ChIP™
EZ-ChIP™ Product

Get tips on using EZ-ChIP™ to perform ChIP Human - Caco-2

Products Merck Millipore EZ-ChIP™
EZ-ChIP™ Product

Get tips on using EZ-ChIP™ to perform ChIP Human - HEK 293

Products Merck Millipore EZ-ChIP™
EZ-ChIP™ Product

Get tips on using EZ-ChIP™ to perform ChIP Human - PANC-1

Products Merck Millipore EZ-ChIP™

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Proteins ChIP Anti-bodies RAD51

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Mouse - RAW264.7

Products Thermo Fisher Scientific Pierce™ Agarose ChIP Kit

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Proteins ChIP Human MDA-MB-231

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