Protein Ladder

Get tips on using CelLytic™ M to perform Protein isolation Bacteria - Staphylococcus aureus

Get tips on using 2-D Quant Kit to perform Protein quantification Colorimetric method

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using PhosphoSerine Antibody Q5 (100 µg) to perform Protein tag Detection of proteins containing phosphorylated serine residues

Get tips on using PhosphoThreonine Antibody Q7 (100 µg) to perform Protein tag Detection of proteins containing phosphorylated threonine residues

Get tips on using Ni-NTA Superflow 96 BioRobot Kit (4) to perform Protein tag Purification of His-tagged proteins

Get tips on using pDG101 to perform Protein Expression Prokaryotic cells - E. coli mScarlet-I

Get tips on using pZH713 to perform Protein Expression Prokaryotic cells - E. coli PP7cp-SYFP2

Get tips on using pFR2 to perform Protein Expression Prokaryotic cells - E. coli ferulic acid

Get tips on using pGETS118 to perform Protein Expression Prokaryotic cells - B. subtilis cellulosomal complexes