Immunohistochemistry Collagen Type VII Rabbit Human

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Get tips on using Purified Rat Anti-Mouse CD24 Clone M1/69 (RUO) to perform Immunohistochemistry Mouse - CD24

Products BD Biosciences Purified Rat Anti-Mouse CD24 Clone M1/69 (RUO)

Get tips on using Purified Rat Anti-Mouse CD24 Clone M1/69 (RUO) to perform Immunohistochemistry Mouse - CD24

Products BD Biosciences Purified Rat Anti-Mouse CD24 Clone M1/69 (RUO)

Get tips on using CD133 (Prominin-1) Monoclonal Antibody (13A4), APC, eBioscience™ to perform Immunohistochemistry Mouse - CD133

Products eBioscience CD133 (Prominin-1) Monoclonal Antibody (13A4), APC, eBioscience™

Get tips on using Mouse GFR alpha-3/GDNF R alpha-3 Antibody to perform Immunohistochemistry Mouse - Gfrα3

Products R&D system, Minneapolis, MN, USA Mouse GFR alpha-3/GDNF R alpha-3 Antibody

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type Rat pulmonary arterial smooth muscle cells

Get tips on using Anti-acetyl-Histone H4 Antibody to perform ChIP acH4 - Rabbit Sheep YFP Tag

Products Millipore Anti-acetyl-Histone H4 Antibody

Get tips on using Recombinant Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572) to perform Immunohistochemistry Mouse - β-Catenin

Products Abcam Recombinant Anti-beta Catenin antibody [E247] - ChIP Grade (ab32572)

Get tips on using Recombinant Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063) to perform Immunohistochemistry Mouse - ERα

Products Abcam Recombinant Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063)

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rabbit aortic smooth muscle cells

Products Thermo Fisher Scientific TRIzol Reagent

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rabbit aortic endothelial cells

Products Qiagen RNeasy Mini Kit

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