Immunohistochemistry Wilms Tumor 1 (WT1) Rabbit

Get tips on using Anti-Collagen Type II Antibody, clone 6B3 to perform Immunohistochemistry Mouse - Col II

Get tips on using β-catenin Antibody (E-5): sc-7963 to perform Immunohistochemistry Mouse - β-Catenin

Get tips on using Human/Mouse/Rat/Canine ALCAM/CD166 Antibody to perform Immunohistochemistry Mouse - CD166 / ALCAM

Get tips on using VENTANA anti-MLH1 (M1) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MLH1

Get tips on using Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 to perform Immunohistochemistry Rat - GFAP

Get tips on using Monoclonal Mouse Anti-Human Hepatocyte (Concentrate) Clone OCH1E5 to perform Immunohistochemistry Mouse - Hepatocyte

Get tips on using Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) to perform Immunohistochemistry Mouse - TUBB3

Get tips on using Recombinant Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917) to perform Immunohistochemistry Mouse - VEGFA

Get tips on using Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker to perform Immunohistochemistry Mouse - PHH3

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.