shRNA gene silencing Human THP-1

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using HyClone RPMI 1640 media: Liquid to perform Mammalian cell culture media THP-1

Get tips on using Gibco™ RPMI 1640 Medium to perform Mammalian cell culture media THP-1

Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using Annexin-V-FLUOS Staining Kit to perform Apoptosis assay cell type - THP-1

Get tips on using ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 Rab Coupling Protein (RCP)

Get tips on using connexin 43 siRNA (r) to perform siRNA / miRNA gene silencing Rat - RMC-1 Cx43

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized THP-1

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized THP-1

Get tips on using YAP siRNA (h) to perform siRNA / miRNA gene silencing Human - OV2008 Yap Gene